H2N2V1, France, Analysis, bibRecord, 000056

Identification of specific packaging signals in influenza A viruses genome

Identifieur interne : 000056 ( France/Analysis ); précédent : 000055; suivant : 000057

Identification of specific packaging signals in influenza A viruses genome

Auteurs : Marie Gerber [France]

Source :

RBID : Hal:tel-01565024

Descripteurs français

mix :
- ARN viraux, Empaquetage du génome, Génétique inverse, Influenzavirus A, Interaction intermoléculaire ARN/ARN.

English descriptors

mix :
- Genome packaging, Influenza A virus, Intermolecular RNA/RNA interaction, Reverse genetics, Viral RNA.

Abstract

The genome of influenza A viruses comprises eight viral RNAs (vRNAs) likely to be selectively packaged into progeny virions as organized supramolecular complexes where vRNAs are held together by base pairing within the packaging signals. To better understand the rules governing this mechanism, we investigated the vRNA/vRNA interaction network of the A/Puerto Rico/8/34 (H1N1) strain. We then identified, at the nucleotide level, using two in vitro approaches, the vRNA sequences involved in several of the interactions. Two of them were functionally tested in an infectious context. We also studied an interaction previously identified in the laboratory between two vRNAs belonging to the circulating A/Moscow/10/99 (H3N2) strain. Finally, we collaborated with Dr L. Brown (Australia) in order to assess the role of an interaction between two vRNAs of the A/Udorn/307/72 (H3N2) strain.

Url:

https://tel.archives-ouvertes.fr/tel-01565024

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000142
to stream Hal, to step Curation: 000142
to stream Hal, to step Checkpoint: 000079
to stream Main, to step Merge: 000371
to stream Main, to step Curation: 000371
to stream Main, to step Exploration: 000371
to stream France, to step Extraction: 000056

Links to Exploration step

Hal:tel-01565024

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of specific packaging signals in influenza A viruses genome</title>
<title xml:lang="fr">Recherche de signaux d'empaquetage spécifiques du génome des virus influenza A</title>
<author><name sortKey="Gerber, Marie" sort="Gerber, Marie" uniqKey="Gerber M" first="Marie" last="Gerber">Marie Gerber</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-93780" status="VALID">          <orgName>Architecture et Réactivité de l'ARN</orgName>
          <orgName type="acronym">ARN</orgName>
          <desc>            <address>              <addrLine>Institut de Biologie Moléculaire et Cellulaire 15 rue René Descartes 67084 STRASBOURG Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www-ibmc.u-strasbg.fr/arn/</ref>
          </desc>
          <listRelation>            <relation active="#struct-1956" type="direct"></relation>
            <relation active="#struct-199013" type="direct"></relation>
            <relation name="FRC1589" active="#struct-441569" type="direct"></relation>
            <relation name="UPR9002" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-1956" type="direct"><org type="regrouplaboratory" xml:id="struct-1956" status="VALID">          <orgName>Institut de biologie moléculaire et cellulaire</orgName>
          <orgName type="acronym">IBMC</orgName>
          <desc>            <address>              <addrLine>I.B.M.C. 15 Rue René Descartes 67084 STRASBOURG CEDEX</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ibmc.u-strasbg.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-199013" type="direct"></relation>
            <relation name="FRC1589" active="#struct-441569" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-199013" type="direct"><org type="institution" xml:id="struct-199013" status="VALID">          <orgName>Université de Strasbourg</orgName>
          <orgName type="acronym">UNISTRA</orgName>
          <desc>            <address>              <addrLine>4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.unistra.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="FRC1589" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UPR9002" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Strasbourg</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Alsace (région administrative)</region>
</placeName>
<orgName type="university">Université de Strasbourg</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:tel-01565024</idno>
<idno type="halId">tel-01565024</idno>
<idno type="halUri">https://tel.archives-ouvertes.fr/tel-01565024</idno>
<idno type="url">https://tel.archives-ouvertes.fr/tel-01565024</idno>
<date when="2016-09-27">2016-09-27</date>
<idno type="wicri:Area/Hal/Corpus">000142</idno>
<idno type="wicri:Area/Hal/Curation">000142</idno>
<idno type="wicri:Area/Hal/Checkpoint">000079</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000079</idno>
<idno type="wicri:Area/Main/Merge">000371</idno>
<idno type="wicri:Area/Main/Curation">000371</idno>
<idno type="wicri:Area/Main/Exploration">000371</idno>
<idno type="wicri:Area/France/Extraction">000056</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of specific packaging signals in influenza A viruses genome</title>
<title xml:lang="fr">Recherche de signaux d'empaquetage spécifiques du génome des virus influenza A</title>
<author><name sortKey="Gerber, Marie" sort="Gerber, Marie" uniqKey="Gerber M" first="Marie" last="Gerber">Marie Gerber</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-93780" status="VALID">          <orgName>Architecture et Réactivité de l'ARN</orgName>
          <orgName type="acronym">ARN</orgName>
          <desc>            <address>              <addrLine>Institut de Biologie Moléculaire et Cellulaire 15 rue René Descartes 67084 STRASBOURG Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www-ibmc.u-strasbg.fr/arn/</ref>
          </desc>
          <listRelation>            <relation active="#struct-1956" type="direct"></relation>
            <relation active="#struct-199013" type="direct"></relation>
            <relation name="FRC1589" active="#struct-441569" type="direct"></relation>
            <relation name="UPR9002" active="#struct-441569" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-1956" type="direct"><org type="regrouplaboratory" xml:id="struct-1956" status="VALID">          <orgName>Institut de biologie moléculaire et cellulaire</orgName>
          <orgName type="acronym">IBMC</orgName>
          <desc>            <address>              <addrLine>I.B.M.C. 15 Rue René Descartes 67084 STRASBOURG CEDEX</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ibmc.u-strasbg.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-199013" type="direct"></relation>
            <relation name="FRC1589" active="#struct-441569" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-199013" type="direct"><org type="institution" xml:id="struct-199013" status="VALID">          <orgName>Université de Strasbourg</orgName>
          <orgName type="acronym">UNISTRA</orgName>
          <desc>            <address>              <addrLine>4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.unistra.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="FRC1589" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UPR9002" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Strasbourg</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Alsace (région administrative)</region>
</placeName>
<orgName type="university">Université de Strasbourg</orgName>
</affiliation>
</author>
</analytic>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Genome packaging</term>
<term>Influenza A virus</term>
<term>Intermolecular RNA/RNA interaction</term>
<term>Reverse genetics</term>
<term>Viral RNA</term>
</keywords>
<keywords scheme="mix" xml:lang="fr"><term>ARN viraux</term>
<term>Empaquetage du génome</term>
<term>Génétique inverse</term>
<term>Influenzavirus A</term>
<term>Interaction intermoléculaire ARN/ARN</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>The genome of influenza A viruses comprises eight viral RNAs (vRNAs) likely to be selectively packaged into progeny virions as organized supramolecular complexes where vRNAs are held together by base pairing within the packaging signals. To better understand the rules governing this mechanism, we investigated the vRNA/vRNA interaction network of the A/Puerto Rico/8/34 (H1N1) strain. We then identified, at the nucleotide level, using two in vitro approaches, the vRNA sequences involved in several of the interactions. Two of them were functionally tested in an infectious context. We also studied an interaction previously identified in the laboratory between two vRNAs belonging to the circulating A/Moscow/10/99 (H3N2) strain. Finally, we collaborated with Dr L. Brown (Australia) in order to assess the role of an interaction between two vRNAs of the A/Udorn/307/72 (H3N2) strain.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Alsace (région administrative)</li>
<li>Grand Est</li>
</region>
<settlement><li>Strasbourg</li>
</settlement>
<orgName><li>Université de Strasbourg</li>
</orgName>
</list>
<tree><country name="France"><region name="Grand Est"><name sortKey="Gerber, Marie" sort="Gerber, Marie" uniqKey="Gerber M" first="Marie" last="Gerber">Marie Gerber</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/France/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000056 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000056 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     Hal:tel-01565024
   |texte=   Identification of specific packaging signals in influenza A viruses genome
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration H2N2

Identification of specific packaging signals in influenza A viruses genome

Identification of specific packaging signals in influenza A viruses genome

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri